RAMbio: Turn your flask into a bioreactor

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Read the Paper: Comparing Culture Characteristics Between Acoustic Resonance Mixing and Orbital Shaking 

Production of recombinant proteins usually happens in suspension cultures, with oxygen limitation playing a major role. We compared growth characteristics of microbial cultures in a standard shaken incubator with those of cultures in a RAMbio fermentation system, paying particular attention to oxygen transfer. The oxygen transfer rates of conventional orbital shakers are insufficient for optimizing microbial fermentations. By improving oxygen transfer as much as sixfold and cell densities as much as threefold, RAMbio systems enable dense cultures in less time than orbital shakers.


The RAMbio maximizes oxygenation in any flask – get more biomass, more protein, more plasmid,
more product.

  • Eliminate oxygen limitations
  • Uses traditional shake flasks
  • Bioreactor-like performance from a simple flask


The RAMbio benchtop incubator uses Resonant Acoustic technology to get maximum performance from microbial cultures, thus replacing orbital shakers.

The RAMbio dramatically enhances oxygenation in bacterial, yeast, and fungal cultures. Using almost any shake flask, you can achieve 6-fold greater gas exchange, meaning you can achieve more than 300% more biomass and significant gains in product production.



Orbital Shakers Suffocate Cultures

Traditional shakers are terrible at aerating microbial cultures. Even with baffled flasks, orbital shakers only allow passive gas exchange. This means that the oxygen supply quickly becomes limited because the oxygen can't enter flasks as fast as the microbes need it.



The RAMbio Lets Your Cultures Breathe

When you use the Oxy-Pump stopper to close your flasks, the RAMbio actually pumps gas in and out of the headspace. Translation: Microbial cultures get all the oxygen they need and reach higher densities than available in orbital shakers.

Fast Growing Aerobes Demand the RAMbio

Whether you're expressing protein, making large quantities of DNA, or just trying to increase biomass yields, the RAMbio is great for culturing strains with high oxygen demands:

  • E. coli
  • Streptomyces sp.
  • Pichia sp.
  • Aspergillus sp.
  • Others...

Resonant Acoustic Mixing is the Key

Unlike the swirling motion of orbital shakers, the RAMbio mixes your culture using low-frequency acoustic energy. Two balanced plates oscillate flasks vertically and provide mixing that improves oxygen transfer into the liquid.

The vertical motion also drives movement of the Oxy-Pump stopper, which actively drives gas exchange with the headspace of the flask, thus eliminating oxygen starvation- a common limitation to culture performance.

 Flask with gas


RAMbio-GraphIf you rely on shake flasks to grow microbes, then you need to explore the RAMbio.

  • 6-fold increase in shake flask oxygen transfer rates

  • Eliminate oxygen limitations for high-demand strains
    - E. coli
    - Pichia sp.
    - Streptomyces sp.
    - Aspergillus sp.

  • Increase product yields
    - Biomass
    - Protein
    - Plasmids and other Nucleic Acids
    - Secondary Metabolites

  • Take advantage of richer medium formulations

  • Humidity control for longer cultivations

System Components

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The RAMbio Incubator


  • 18 x 250mL flasks
  • 14 x 500mL flasks
  • 8 x 1,000mL flasks

Control Features

  • Temperature: ambient +5˚C to 60˚C
  • Humidity: ambient to 90% RH
  • Timed setpoint profiles possible


  • Dimensions (W/D/H): 102 x 56 x 69cm
  • Power: 110/230 VAC, 50/60 Hz
  • Weight: 190kg

The Oxy-Pump Stopper

  • Fits virtually any shake flask
  • Fully re-usable
  • Autoclavable
  • Enables maximum gas transfer

Request more information about the RAMbio Incubator



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