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Ovizio 10iLine Fast On-Line Quantitative Cell Imaging Analyzer

NEW! Read the article at The Cell Culture Dish and watch a webnar on using the iLine F to monitor viral load in real time in cell culture.

General

The iLine Fast imaging technology is based on patented Differential Digital Holographic Microscopy. This unique technology brings real-time label free monitoring to suspension cell bioreactors. In Digital Holography, the scattered light beam from an illuminated object interferes with a reference beam on a CCD camera allowing for a 3D numerical reconstruction of the object. 

Differential digital holography is an evolution of this base technology that brings increased stability and an important size reduction of the instruments.
Digital Holography can construct intensity images, quantitative phase images and 3D images covering the shape and density of an object in a volume. The phase image is equivalent to the optical thickness of the sample. Images of cells captured in a hologram can be refocused after acquisition.
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The iLine Fast measures the specific holographic signature of each cell within the field of view and computes a cell count based on a sequence of holograms. The platform shows cell density and cell viability per measurement sequence. 

The iLine fast is designed specifically for use in combination with bioreactors. The technology can track a full cycle and can count cell viability from very low to high concentrations.
The iLine Fast can be used in various settings and can be integrated with the bioreactor control systems.

 Features

This device has been developed for direct monitoring of suspension bioreactors. The device has built-in functionality to monitor cell density and cell viability in real-time.

The iLine Fast can be connected to 4 bioreactors via an innovative disposable fluidics system.
The advanced, easy to use software captures real time 4D images and quantitative data allowing for automatic cell counting and other specific measurements.
The total magnification is typically 20 X but can be adapted to fit the size of the cells to be observed.

MEASUREMENTS

  • Cell count
  • Cell viability
  • Cell morphology
  • Cell clusters

The technology can be used throughout the bioreactor cycle and will allow the operator to track cell density and cell viability in real-time eliminating the need for sampling and reducing the risk of contamination. Tracking cell growth continuously allows for accurate prediction of optimum harvest times.

The platform is designed to cope with very low cell densities and very high densities. 
Quantitative imaging as a new Process Analytical Technology (PAT) applied to on-line bioreactor monitoring improves process knowledge, quality and productivity in R&D, Pilot and Production environments.

FEATURES

  • On-line bioreactor monitoring
  • Viable cell density measurement
  • Label-free
  • Reduced operational costs up to 50%
  • No sampling, no sample preparation
  • Non-invasive
  • Environmental-friendly (no waste)

Software

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The iLine Fast is delivered in combination with an easy to operate software package. This workflow driven application is intended to get beyond the burden of current cumbersome life sciences instrument applications and is focused on giving the user a straightforward experience.
OsOne allows the user to focus on the result of a measurement while offering all the tools required for a thorough investigation of the underlying data.

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Operation

The iLine Fast is connected to bioreactors via a specific, sterile closed loop disposable fluidics system. Cells are pumped out of the bioreactor, via an innovative pumping system, flow through the imaging device in a temperature-controlled environment and flow back to the bioreactor after the holograms of the cells have been captured. The holograms are continuously analyzed to compute cell density and cell viability.

Thanks to its on-line features, the technology strongly improves productivity and quality and allows staff in charge of the bioprocess environment to focus on tasks not linked to sampling, staining and analyses of data.
The monitoring platform is used continuously throughout the cell multiplication cycle and gives very accurate information related to the ideal harvest time of the culture.

CELL DENSITY AND VIABILITY
Cell viability and density are indicated via a simple visual gauge. For more in depth knowledge, Individual cells can be observed and analyzed via an easy to use cell browser. Green dots represent living cells, red dots represent dead cells and yellow dots represent cells within an aggregate.

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Cell density and viability are measured by using a label free method based on the “holographic” fingerprint of a cell. First of all the iLine Fast captures holograms of all the cells flowing through the fluidics system in a 3D volume. In a second phase all objects within the observed volume, typically 100 times larger than traditional microscopes, are refocused. In a third step the holographic fingerprint of each cell is computed. The fingerprint is computed by an expert system and is based on a combination of parameters that allow for a fast and accurate classification. The methodology used by the quantitative imaging based platform can distinguish living and death cells and can eliminate debris easily making it a versatile and cost effective solution.

Ovizio’s platform has been benchmarked and compared to existing manual and automated off-line methods and has shown to be very consistent and accurate. 

Measurements

Cell viability and density are indicated via a simple visual gauge. For more in depth knowledge, Individual cells can be observed and analyzed via an easy to use cell browser. Green dots represent living cells, red dots represent dead cells and yellow dots represent cells within an aggregate.

image007   image008

Cell density and viability are measured by using a label free method based on the “holographic” fingerprint of a cell. First of all the iLine Fast captures holograms of all the cells flowing through the fluidics system in a 3D volume. In a second phase all objects within the observed volume, typically 100 times larger than traditional microscopes, are refocused. In a third step the holographic fingerprint of each cell is computed. The fingerprint is computed by an expert system and is based on a combination of parameters that allow for a fast and accurate classification. The methodology used by the quantitative imaging based platform can distinguish living and death cells and can eliminate debris easily making it a versatile and cost effective solution.

Ovizio’s platform has been benchmarked and compared to existing manual and automated off-line methods and has shown to be very consistent and accurate. 

Learn More

  • Webinar and Article: In-Line Viral Load Measurement using Smart Cell Culture Monitoring - video and article

Request more information about the iLine-F Analyzer from your local Applikon representative.

Request more information about the iLine F

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